（1）报告人姓名：谢德欢
（2）报告题目：Comparative Analysis of Liver Metabolomics and Histopathology in Mice Following Ultrahigh Dose-Rate (FLASH) and Conventional Irradiation
（3）报告人简介：广东省人民医院放疗科医师，至今发表SCI论文10余篇，主持国家自然科学基金青年项目1项，参与横向课题2项，主要从事FLASH放射损伤研究、靶区勾画及剂量处方优化研究。
（4）报告内容简介：
Purpose: Ultrahigh dose-rate (FLASH) irradiation has garnered attention for its potential to reduce damage to normal tissues while maintaining anti-tumor efficacy. However, the specific impact of FLASH on liver tissue and its metabolic profile remains poorly understood, necessitating further investigation.
Methods and Materials: Female BALB/c mice were assigned to receive a single dose of 20 Gy FLASH or CONV irradiation. Animals were monitored for 4 or 20 days post-irradiation, after which plasma and liver tissues were collected. Serum biochemistry, histopathological assessment, and untargeted metabolomics using LC-MS platforms were performed.
Results: FLASH irradiation was associated with accelerated body weight recovery and reduced liver damage in histopathological evaluations. Serum levels of liver enzymes ALT and AST were markedly higher in the CONV group at 20 days post-irradiation, suggesting greater liver injury. Metabolomics profiling identified 293 differential metabolites between FLASH and CONV groups, with significant lipid-related metabolic changes. Gene Set Enrichment Analysis (GSEA) indicated downregulation of pathways involved in unsaturated fatty acid and tryptophan metabolism, and upregulation of nucleotide, sphingolipid signaling, and pyruvate metabolism in the FLASH group.
Conclusion: Our findings provide novel insights into the metabolic adjustments that may contribute to the liver's enhanced tolerance to FLASH irradiation. The comprehensive metabolomics evaluation on liver tissue provides new ideas for clarifying the mechanisms behind FLASH effect, promoting the clinical translation of FLASH irradiation technology.